1. Product Overview
The ABN UVMK leverages coherent Raman scattering (including SRS/CARS) as its core technology, combined with multimodal nonlinear optical imaging techniques, to achieve label-free, chemically specific, high-resolution imaging of biological and material samples. Its key strengths include a million-fold improvement in sensitivity, video-rate imaging speed, submicron spatial resolution and multimodal co-localization capability. It addresses the challenges of phototoxicity, photobleaching and small-molecule labeling limitations inherent to conventional fluorescence imaging, while overcoming the bottlenecks of weak spontaneous Raman signals and slow imaging speed. It supports full-scenario research and application requirements ranging from dynamic metabolic studies of living cells to the analysis of material microstructures. As a coherent Raman scattering microscopy imaging technology built on nonlinear optical effects, it integrates polymer specificity, high sensitivity, high spatial resolution and fast imaging speed. It fully meets cutting-edge demands in fields such as life sciences and materials science, boasting distinctive technical advantages and broad application prospects.
II. Equipment Specifications
| Parameter Category | Specific parameters |
|---|
| Core technology | Coherent Raman Scattering (SRS/CARS), Two-Photon Excited Fluorescence (TPEF), Second Harmonic Generation (SHG), Transient Absorption (TA) |
| laser system | Channel 1: 1045 nm, Channel 2: 680-1300 nm; Pulse width < 200 fs, Repetition rate 80 MHz |
| Spectral Performance | Spectral range: fingerprint region 800–1800 cm⁻¹, C-D bond 2000–2200 cm⁻¹, C-H bond 2800–3000 cm⁻¹; spectral resolution ＜17 cm⁻¹ (customizable) |
| Imaging performance | Spatial resolution ≤350 nm; maximum imaging speed of 10 fps; supports 2D/3D imaging and large-field stitching |
| Objective Lens Configuration | Standard configuration: 20×/40×/60×; high-NA water immersion objectives / oil immersion objectives are optional, compatible with different sample thicknesses and resolution requirements |
| software system | Supports multimodal image fusion, MCR multivariate curve analysis, and quantitative data export (Excel format) |
| Environmental Requirements | Temperature: 22–25°C, Humidity: 40%–60%, equipped with a dedicated vibration isolation table（2400×1500 mm） |
III. Product Features
Label-free Chemical Specific Imaging: Based on molecular vibrational fingerprints, it requires no fluorescent staining, avoids phototoxicity and dye interference, and preserves the physiological activity of samples.
Million-fold Sensitivity and Video-rate Speed: The sensitivity is enhanced by 10⁶ times compared with spontaneous Raman. It supports imaging at up to 10 fps, enabling capture of dynamic metabolic processes in live cells.
Multimodal Colocalization: Simultaneously acquires CRS, TPEF, SHG and TA signals to realize multi-dimensional correlation analysis of structure-function-chemical composition.
Submicron Resolution and 3D Imaging: It achieves a spatial resolution of ≤350 nm, supports dynamic focus compensation and 3D stereoscopic imaging, and is suitable for analysis of thick samples and microstructures.
Flexible Spectroscopy and Data Processing: Multi-window spectral scanning (fingerprint region / C-D bond / C-H bond), built-in MCR quantitative analysis, and one-click data export and statistics are available.
IV. Technical Advantages
Coherent Raman Enhancement Technology: SRS/CARS resonantly amplifies signals, boosting sensitivity by 10⁶ times relative to spontaneous Raman, and high-quality Raman images can be acquired within 1 second.
Integrated Multimodal Nonlinear Optics: Seamless integration of CRS with TPEF/SHG/TA. A single scan obtains structural, functional and chemical information, increasing data dimensionality and analysis efficiency.
High-speed Galvo Scanning System: The 2D galvanometer provides a refresh rate up to 10 fps, supporting real-time monitoring of dynamic processes in live cells such as lipid metabolism and protein synthesis.
Precise Spectral and Spatial Control: Broad spectral coverage (800–3000 cm⁻¹) and ≤350 nm resolution, suitable for analysis of biomolecules including small molecules, lipids and proteins, as well as material microstructures.
Intelligent Data Processing and Analysis: MCR multivariate curve resolution automatically identifies mixed components and quantitatively outputs concentration distribution. One-click data export and statistics reduce barriers to analysis.
V. Application Scenarios and Fields
Life Science Research
Cell Metabolism: Label-free real-time monitoring of dynamic changes of lipids, proteins and nucleic acids in living cells, suitable for metabolic tracking of model organisms such as Caenorhabditis elegans.
Synthetic Biology: Visualization of enzyme-catalyzed processes and metabolic pathways, label-free imaging of small-molecule metabolites to avoid interference from fluorescent labeling.
Botany: Distribution of leaf cuticle and cell wall components, dynamic monitoring of oil accumulation in seeds without sectioning and staining.
Medical Pathology and Diagnosis
Oncology Research: Abnormal distribution of lipids and proteins in tumor tissues, label-free pathological classification to assist early diagnosis.
Metabolic Diseases: Imaging of lipid deposition in fatty liver and atherosclerosis to assess lesion severity and therapeutic effects.
Ultra-Multiplex Immunohistochemistry: Combined with immunofluorescence to realize co-localization analysis of multi-target proteins and metabolites.
Materials Science and Chemical Analysis
Polymer Materials: Phase separation of blends and component distribution of thin-film coatings, label-free characterization of microstructures and interfacial properties.
Electrochemical Materials: Composition of SEI films on battery electrode interfaces and microstructures of solid electrolytes, compatible with in-situ imaging inside anhydrous and oxygen-free glove boxes.
Nanomaterials: Dispersibility of nanoparticles and interlayer interactions of two-dimensional materials, chemically specific analysis at submicron resolution.
Drug Research and Formulation
Drug Delivery: In vitro and in vivo distribution of liposomes and nanomicelles, label-free tracking of drug release and metabolic pathways.
Formulation Analysis: Drug crystal forms and excipient distribution, evaluation of formulation stability and bioavailability.
Environmental and Food Science
Microbial Communities: Metabolic activities of anaerobic microorganisms, label-free observation of the relationship between community structure and functions.
Food Components: Distribution of lipids, proteins and carbohydrates, rapid adulteration detection and quality control.
