I. Product Overview

An inverted fluorescence microscope is a high-performance fluorescence imaging instrument featured with an inverted optical structure. With objectives positioned below the sample and the illumination & detection systems arranged above it, it is applicable to high-sensitivity fluorescence imaging and analysis of living cells, tissue specimens and functional materials.

Capable of multi-channel fluorescence signal acquisition and quantitative analysis without disrupting the sample culture environment, this equipment is widely used in life sciences, biomedicine, pharmaceutical R&D and interdisciplinary materials research. It has become one of the indispensable core imaging platforms in modern scientific research laboratories.

Technical Parameters

1. Objective Lens: 4×, 10×, 20×, 40×

2. Eyepiece: Wide-field flat-field eyepiece WF10X; Field of view: Φ22 mm; Eyepiece interface: Φ30 mm

3. Color Fluorescence Camera: 12.5 million pixels, equipped with USB3.0 data cable; Maximum frame rate at full resolution: 15 frames/s; 1-inch sensor chip; Spectral response range: 380–1100 nm

4. Adaptor: Stage adapters for cell culture plates, 35 mm petri dishes, 60 mm petri dishes, 90 mm cell culture dishes and glass slides

5. Cold Light Source: LED cold light source with continuously adjustable brightness

6. Host Computer: Intel Core i5, 16G RAM + 256G SSD

7. Filter: Ground glass, blue filter and green filter

II. Functional Features

• Inverted fluorescence optical system: Adopts a mature and reliable inverted optical path design. The objective lenses are placed below the stage, enabling direct observation of thick samples such as petri dishes and multi-well plates.

• Multi-channel fluorescence imaging capability: Supports the configuration of various fluorescence excitation and emission channels, covering commonly used blue, green, red and other wavelength bands to meet the requirements of multi-labeling experiments.

• High-sensitivity signal acquisition: Equipped with high-performance fluorescence filter sets and scientific-grade imaging interfaces, it realizes stable detection of weak fluorescence signals.

• Stable and reliable stage and focusing structure: The stage features high load-bearing capacity, and the focusing mechanism delivers smooth operation, suitable for long-term imaging experiments.

• Excellent system expandability: It supports the expansion of multiple functional modules such as cameras, motorized stages and environmental control units, facilitating subsequent system upgrading and integration.

III. Application Scenarios and Fields

• Analysis of cell morphology, organelle distribution and fluorescent protein localization in cell biology research;

• Fluorescence detection and quantitative analysis of tumor cells and immuno-labeled samples in biomedical research;

• Multi-well plate fluorescence imaging and drug efficacy evaluation in drug research and development as well as high-throughput screening experiments;

• Long-term, low-phototoxicity fluorescence imaging observation of dynamic processes in living cells;

• Optical performance characterization and comparative analysis of fluorescent functional materials and biomaterials.

IV. Technical Advantages

• Mature optical system design with stable fluorescence imaging performance. The inverted fluorescence microscope adopts a well-developed fluorescence optical path and filter system configuration. While ensuring imaging resolution and contrast, it effectively suppresses stray light interference, providing a reliable optical foundation for highly reproducible fluorescence experiments.

• Reasonable structural design and high long-term operational stability. The overall layout of the mechanical structure and optical components fully takes into account system rigidity and thermal stability, which can effectively reduce the impact of environmental vibration and temperature changes on image quality. It is suitable for long-term continuous imaging and time-lapse experimental applications.

• Strong multi-channel fluorescence compatibility and high experimental flexibility. The system supports combined use and fast switching of multiple fluorescence channels. Different fluorescent labeling schemes can be flexibly configured according to experimental requirements to meet the multi-parameter observation needs of complex biological samples.

• Compatible with automation and software control to improve experimental efficiency. Optional motorized control and image acquisition & analysis software are available to realize automatic focusing, automatic scanning and data recording. It effectively reduces manual operation errors and improves experimental repeatability and working efficiency.

• Meeting both scientific research and engineering application needs with a wide range of applicable scenarios. While maintaining high-performance fluorescence imaging capability, the equipment features user-friendly operation and convenient maintenance. It is suitable not only for basic scientific research, but also for experimental requirements in application development and engineering verification stages.

V. Typical Application Cases

Note: The above configurations can be customized and adjusted according to users' experimental requirements.

Sample Image Observed by Abner Inverted Fluorescence Microscope

Equipment Appearance Drawing